|Titre :||Human Milk Adrenomedullin Is Unstable During Cold Storage at 4°C (2017)|
|Auteurs :||Chiara Peila, Auteur ; Alessandra Coscia, Auteur ; Enrico Bertino, Auteur ; Giovanni Li Volti, Auteur ; Fabio Galvano, Auteur ; Ignazio Barbagallo, Auteur ; Diego Gazzolo, Auteur|
|Type de document :||Article : texte imprimé|
|Dans :||Breastfeeding Medicine (Vol 12, n°9, Novembre 2017)|
|Article en page(s) :||pp. 561-565|
|Mots-clés:||bioactivité, adrenomedulline, réfrigération|
"Introduction: Under some circumstances human milk (HM) extraction and refrigerated storage may be necessary. Depending on the length and on the type of cold storage, milk may lose some important properties, but current advices on safe HM storage are discordant. Moreover until now no data in literature were present on the effect of prolonged cold storage on biologically active components of the HM such as adrenomedullin (AM). This important peptide is involved in response to hypoxia and inflammation, associated with neovascularization, in several tissues. The aim is to evaluate: (a) the presence of AM in preterm and term HM and (b) the concentration of AM in refrigerated milk at 4°C at 24-hour intervals, up to 96 hours of storage.
Materials and Methods: The experiment was repeated four times. Immediately after collection, each HM sample deriving from each mother was divided into two parts as follows: Pool line and Single Mother line. One part (Pool line) was pooled and then divided into five aliquots. The other part (Single Mother line) was divided into five aliquots. From each line, one aliquot was analyzed within 3 hours, while the others were stored in the refrigerator for 24, 48, 72, and 96 hours, respectively, and then analyzed. AM levels were determined using a specific ELISA test.
Results: AM was detectable in all samples. Its concentration was significantly higher in preterm milk with respect to term milk (p
Discussion: This study provides evidences regarding the presence of AM in HM, regardless of the gestational age. In particular, the refrigeration of fresh HM in controlled conditions significantly affected its bioactivity and nutritional quality related with AM, already at 24 hours."[résumé de l'auteur]